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简要描述:BCDF Elisa试剂盒可检测血清、血浆、细胞培养上清或其它相关生物液体中的含量或者活性表达。进口试剂盒货期短,物流安全迅速,检测数据准确,是您实验*,免费为客户提供全面技术咨询服务及相关产品中英文双版说明书,技术服务:专业,及时,耐心。
产品型号: 96T
所属分类:ELISA试剂盒进口
更新时间:2016-02-22
BCDF Elisa试剂盒是一种敏感性高,特异性强,重复性好的实验诊断方法,由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检。
灵敏度,检测范围,特异性和重复性:
灵敏度:zui小可测 0.094ng/mL。
检测范围:0.156–10ng/mL。
特异性:可检测重组或天然的待测物质,且与其它相关蛋白无交叉反应。
重复性:板内,板间变异系数均<10%。
BCDF ELISA分析检测试剂盒,英文名称:BCDF ELISA Kit,常用网络搜索名:BCDF ELISA试剂盒,规格:48T/96T。
BCDF Elisa试剂盒间接法测抗体流程示意图:
BCDF Elisa试剂盒样本稀释原则:
首先通过文献检索的方式了解待测样本的大致含量,确定适当的稀释倍数。只有稀释至标准曲线的范围内,检测的结果才是准确的。稀释的过程中,应做好详细的记录。zui后计算浓度时,稀释了“N”倍,标本的浓度应再乘以“N”。
(1)、标准品的稀释原则:2瓶,每瓶临用前以样品稀释液稀释至1ml,盖好后静置10分钟以上,然后反复颠倒/搓动以助溶解,其浓度为300 pg/ml,做系列倍比稀释后,分别稀释300 pg/ml,150 pg/ml,75 pg/ml,37.5 pg/ml,18.5 pg/ml,9 pg/ml,4.5 pg/ml,样品稀释液直接作为标准浓度0 pg/ml,临用前15分钟内配制。
如配制150 pg/ml标准品:取0.5ml(不要少于0.5ml)300 pg/ml的上述标准品加入含0.5ml样品稀释液的Eppendorf管中,混匀即可,其余浓度以此类推。
(2)、BCDF Elisa试剂盒*标记抗体的稀释原则:临用前以*标记抗体稀释液稀释,稀释前根据预先计算好的每次实验所需的总量配制(每孔100μl),实际配制时应多配制0.1-0.2ml。如10μl*标记抗体加990μl*标记抗体稀释液的比例配制,轻轻混匀,在使用前一小时内配制。
(3)、辣根过氧化物酶标记亲和素的稀释原则:临用前以辣根过氧化物酶标记亲和素稀释液稀释,稀释前根据预先计算好的每次实验所需的总量配制(每孔100μl),实际配制时应多配制0.1-0.2ml。如10μl辣根过氧化物酶标记亲和素加990μl辣根过氧化物酶标记亲和素稀释液 的比例配制,轻轻混匀,在使用前一小时内配制。
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