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简要描述:小鼠EGF蛋白(His标签)收到后首先观察细胞瓶是否完好,培养液是否有漏液、浑浊等现象,若有上述现象发生请及时和我们。仔细阅读细胞说明书,了解细胞相关信息,如细胞形态、所用培养基、血清比例、所需细胞因子等。
产品型号: 美国ATCC
所属分类:细胞因子
更新时间:2016-01-27
小鼠EGF蛋白(His标签)保证运输中细胞的正常生长,关于其价格、报价、货期,请咨询我们。
特点:细胞代数为2-3代左右。
细胞用途:提供用于科研的稳定细胞系。
细胞纯度:92%
细胞活力:88%(ViabilitybyTrypanBlueExclusion)。
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。
储存方法:液氮(冻存)或复苏培养。
规格:500000cells/瓶
小鼠EGF蛋白(His标签)实验结果
1.定期观察细胞培养过程中集落的形成。
2.显微镜下计数大于50个细胞克隆数,然后按下式计算集落形成率:
集落形成率(%)=(集落数/接种细胞数)×100
小鼠EGF蛋白(His标签)注意事项
1.琼脂对热和酸不稳定,如果反复加热,容易降解,产生毒性,同时琼脂硬度下降。故琼脂高压灭菌( 10磅 15分钟)后按一次用量进行分装。
2.细胞悬液中,细胞分散度>95%。
3.软琼脂培养时,注意琼脂与细胞混合时温度不要超过 40℃ ,以免烫伤细胞。
4.接种细胞密度不宜过高。
5.小鼠EGF蛋白(His标签)在低密度条件下培养,生存率明显下降,无限细胞系和肿瘤细胞株克隆形成率一般在10%以上。但初代培养细胞和有限细胞系仅为0.5~5%,甚至为零。为提高集落形成率,必要时在培养基中添加胰岛素、*等促细胞克隆形成物质。
xy1381 bFGF-std MEF-cm 碱性成纤维细胞生长因子刺激的小鼠胚胎成纤维细胞条件培养基 bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium 100ml
xy1382 CEpiCM 角膜上皮细胞培养基 Corneal Epithelial Cell Medium 500 ml
xy1383 OEpiCM 卵巢上皮细胞培养基 Ovarian Epithelial Cell Medium 500 ml
xy1384 MSCM-sf 间充质干细胞培养基 Mesenchymal Stem Cell Medium-serum free 500 ml
xy1385 MEpiCM 乳腺上皮细胞培养基 Mammary Epithelial Cell Medium 500 ml
xy1386 KM-acf 角质细胞培养基 Keratinocyte Medium-animal component free 500 ml
xy1387 FM-acf 成纤维细胞培养基 Fibroblast Medium-animal component free 500 ml
xy1388 STEMium-ACF 人类多能干细胞培养基-无动物组分 STEMium Human Pluripotent Stem Cell Growth Medium-animal component free 500ml
xy1389 MSCM-acf 间充质干细胞培养基 Mesenchymal Stem Cell Medium-animal component free 500 ml
xy1390 MEM MEM MEM with Earle's Salts and L-Glutamine 500 ml
xy1391 MEM MEM MEM with Hank's Salts and L-Glutamine 500 ml
xy1392 MEM MEM MEM with Eagles's Salts and Glutamine 500 ml
xy1393 M199E M199E Medium 199 with Earle's Salts and L-Glutamine 500 ml
xy1394 M199H M199H Medium 199 with Hanks Salts and L-Glutamine 500 ml
xy1395 DMEM DMEM DMEM with L-Glutamine and Sodium Pyruvate 500 ml
xy1396 DMEM DMEM DMEM with High-Glucose - L-Glutamine and Sodium Pyruvate 500 ml
xy1397 DMEM DMEM DMEM with High-Glucose - L-Glutamine and Sodium Pyruvate 1L
xy1398 DMEM DMEM DMEM with L-Glutamine - Sodium Pyruvate and 25 mM HEPES 500 ml
xy1399 DMEM DMEM DMEM with High-Glucose - L-Glutamine - Sodium Pyruvate and 25 mM HEPES 500 ml
xy1400 DMEM-prf DMEM-prf DMEM with High Glucose - L-Glutamine and Sodium Pyruvate - phenol red-free 1L
xy1401 Ham's F-10 Ham's F-10 F-10 with L-Glutamine and 25 mM HEPES 500 ml
xy1402 Ham's F-12 Ham's F-12 F-12 with L-Glutamine and 25 mM HEPES 500 ml
xy1403 DMEM/F-12 DMEM/F-12 DMEM/F-12 with L-Glutamine 500 ml
xy1404 DMEM/F-12 DMEM/F-12 DMEM/F-12 with L-Glutamine and 15 mM HEPES 500 ml
xy1405 RPMI 1640 RPMI 1640 RPMI 1640 without L-Glutamine - with 25 mM HEPES 500 ml
xy1406 RPMI 1640 RPMI 1640 RPMI 1640 with L-Glutamine and 25 mM HEPES 500 ml
xy1407 IMDM IMDM MDM with L-Glutamine and 25 mM HEPES; without alpha-Thioglycerol - 2-mercaptoethanol 500 ml
xy1408 rhSCF 重组人干细胞因子 Recombinant Human Stem Cell Factor 2μg
xy1409 rhG-CSF 重组人粒细胞集落刺激因子 Recombinant Human Granulocyte Colony Stimulating Factor 2μg