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简要描述:人胰岛素样生长因子-2检测试剂盒适用于体外定量检测人血清、血浆或其它相关生物液体中天然和重组胰岛素样生长因子-2的浓度。本试剂盒仅供体外研究使用、不用于临床诊断!
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所属分类:Human/人检测试剂盒
更新时间:2015-11-05
人胰岛素样生长因子-2检测试剂盒在ELISA检测系统的关键要素中,包被原的性质很重要,蛋白浓度,是否降解,这关系到你做出的抗体可不可以被其识别,所以保留抗原很重要,ELISA试剂盒我做重组蛋白时,一定要在冰浴下缓慢融化就是这个道理。封锁就是让大量不相关的蛋白质充填这些旷地空闲,从而排斥ELISA后的步骤中干扰物质的再吸附。但有时因为试验的需要,包被原的特殊性也可能采用中性的缓冲溶液来包。
【中文名称】人胰岛素样生长因子-2检测试剂盒【常用名称】人胰岛素样生长因子-2ELISA试剂盒【规格】96T/48T【品牌】TSZ【价格】电议【保存】2-8℃,避光保存【说明书】随货发送或直接销售人员索取【有效期】六个月【检测目的】用于测定血清,血浆及相关液体等样本,例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本
注:本产品仅供科研使用,不得用于临床诊断
人胰岛素样生长因子-2检测试剂盒实验内容与方法
1.在微量反应板每孔加入待检标本50μl,设阳性、阴性对照各2孔,每孔加入阳性(或阴性)对照各1滴,并设空白对照1孔。
2.每孔加入酶结合物1滴(空白对照除外),充分混匀,封板,置37℃孵育30分钟。
3.弃去孔内液体,洗涤液注满各孔,静置5秒,甩干,重复5次后拍干。
4.每孔加显色剂A液、B液各1滴,充分混匀,封板,置37℃孵育15分钟。
5.每孔加终止液1滴,充分混匀。
6.用酶标仪读数,取波长450nm,先用空白孔校零,然后读取各孔OD值。
人胰岛素样生长因子-2检测试剂盒相关现货试剂盒如下:xyA108Ga 鸡骨保护素(OPG)ELISA试剂盒 ELISA Kit for Osteoprotegerin (OPG) 检测范围:14.326-1000pg/ml
xyA214Ga 鸡窖蛋白(CAV1)ELISA试剂盒 ELISA Kit for Caveolin 1 (CAV1) 检测范围:14.326-1000pg/ml
xyA135Ga 鸡血小板生成素(TPO)ELISA试剂盒 ELISA Kit for Thrombopoietin (TPO) 检测范围:14.326-1000pg/ml
xyA147Ga 鸡脂联素受体1(ADIPOR1)ELISA试剂盒 ELISA Kit for Adiponectin Receptor 1 (ADIPOR1) 检测范围:14.326-1000pg/ml
xyA164Ga 鸡泛素(Ub)ELISA试剂盒 ELISA Kit for Ubiquitin (Ub) 检测范围:14.326-1000pg/ml
xyA063Ga 鸡白介素17(IL17)ELISA试剂盒 ELISA Kit for Interleukin 17 (IL17) 检测范围:14.326-1000pg/ml
xyC470Ga 鸡脂肪酸合酶(FASN)ELISA试剂盒 ELISA Kit for Fatty Acid Synthase (FASN) 检测范围:14.326-1000pg/ml
xyA049Gu ELISA Kit for Interferon Gamma (IFNg) 检测范围:15.625-1000pg/ml
xyA482Gu ELISA Kit for Endothelin 1 (EDN1) 检测范围:15.625-1000pg/ml
xyA133Gu ELISA Kit for Tumor Necrosis Factor Alpha (TNFa) 检测范围:15.625-1000pg/ml
xyA124Gu ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1) 检测范围:15.625-1000pg/ml
xyA207Gu ELISA Kit for Alanine Aminotransferase (ALT) 检测范围:15.625-1000pg/ml
xyA821Gu ELISA Kit for C Reactive Protein (CRP) 检测范围:15.625-1000pg/ml
xyA563Gu ELISA Kit for Interleukin 1 Beta (IL1b) 检测范围:15.625-1000pg/ml
xyA080Gu ELISA Kit for Interleukin 8 (IL8) 检测范围:15.625-1000pg/ml
xyA056Gu ELISA Kit for Interleukin 10 (IL10) 检测范围:15.625-1000pg/ml
xyA078Gu ELISA Kit for Interleukin 5 (IL5) 检测范围:15.625-1000pg/ml
xyA104Gu ELISA Kit for Cholecystokinin A Receptor (CCKAR) 检测范围:15.625-1000pg/ml
xyA103Gu ELISA Kit for Matrix Metalloproteinase 8 (MMP8) 检测范围:15.625-1000pg/ml
xyA116Gu ELISA Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) 检测范围:15.625-1000pg/ml
xyC011Gu ELISA Kit for Complement Factor B (CFB) 检测范围:15.625-1000pg/ml
xyA073Gu ELISA Kit for Interleukin 2 (IL2) 检测范围:15.625-1000pg/ml
xyA033Gu ELISA Kit for Interferon Alpha (IFNa) 检测范围:15.625-1000pg/ml
xyA051Gu ELISA Kit for Insulin Like Growth Factor 2 (IGF2) 检测范围:15.625-1000pg/ml
xyA128Gu ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 检测范围:15.625-1000pg/ml
xyB949Gu ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3) 检测范围:15.625-1000pg/ml
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