全国统一服务热线:15221858802
简要描述:人胰岛素样生长因子-Ⅰ检测试剂盒适用于体外定量检测人血清、血浆或其它相关生物液体中天然和重组胰岛素样生长因子-Ⅰ的浓度。本试剂盒仅供体外研究使用、不用于临床诊断!
产品型号:
所属分类:Human/人检测试剂盒
更新时间:2015-11-05
人胰岛素样生长因子-Ⅰ检测试剂盒在ELISA检测系统的关键要素中,包被原的性质很重要,蛋白浓度,是否降解,这关系到你做出的抗体可不可以被其识别,所以保留抗原很重要,ELISA试剂盒我做重组蛋白时,一定要在冰浴下缓慢融化就是这个道理。封锁就是让大量不相关的蛋白质充填这些旷地空闲,从而排斥ELISA后的步骤中干扰物质的再吸附。但有时因为试验的需要,包被原的特殊性也可能采用中性的缓冲溶液来包。
【中文名称】人胰岛素样生长因子-Ⅰ检测试剂盒【常用名称】人胰岛素样生长因子-ⅠELISA试剂盒【规格】96T/48T【品牌】TSZ【价格】电议【保存】2-8℃,避光保存【说明书】随货发送或直接销售人员索取【有效期】六个月【检测目的】用于测定血清,血浆及相关液体等样本,例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本
注:本产品仅供科研使用,不得用于临床诊断
人胰岛素样生长因子-Ⅰ检测试剂盒实验内容与方法
1.在微量反应板每孔加入待检标本50μl,设阳性、阴性对照各2孔,每孔加入阳性(或阴性)对照各1滴,并设空白对照1孔。
2.每孔加入酶结合物1滴(空白对照除外),充分混匀,封板,置37℃孵育30分钟。
3.弃去孔内液体,洗涤液注满各孔,静置5秒,甩干,重复5次后拍干。
4.每孔加显色剂A液、B液各1滴,充分混匀,封板,置37℃孵育15分钟。
5.每孔加终止液1滴,充分混匀。
6.用酶标仪读数,取波长450nm,先用空白孔校零,然后读取各孔OD值。
人胰岛素样生长因子-Ⅰ检测试剂盒相关现货试剂盒如下:xyA292Ga 鸡α-胞衬蛋白(SPTAN1)ELISA试剂盒 ELISA Kit for Alpha-Fodrin (SPTAN1) 检测范围:14.326-1000pg/ml
xyA008Ga 鸡血管生成素1(ANGPT1)ELISA试剂盒 ELISA Kit for Angiopoietin 1 (ANGPT1) 检测范围:14.326-1000pg/ml
xyA011Ga 鸡脑源性神经营养因子(BDNF)ELISA试剂盒 ELISA Kit for Brain Derived Neurotrophic Factor (BDNF) 检测范围:14.326-1000pg/ml
xyA010Ga 鸡结缔组织生长因子(CTGF)ELISA试剂盒 ELISA Kit for Connective Tissue Growth Factor (CTGF) 检测范围:14.326-1000pg/ml
xyA013Ga 鸡骨成型蛋白2(BMP2)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 2 (BMP2) 检测范围:14.326-1000pg/ml
xyA014Ga 鸡骨成型蛋白4(BMP4)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 4 (BMP4) 检测范围:14.326-1000pg/ml
xyA021Ga 鸡睫状神经营养因子(CNTF)ELISA试剂盒 ELISA Kit for Ciliary Neurotrophic Factor (CNTF) 检测范围:14.326-1000pg/ml
xyA017Ga 鸡上皮性钙黏附蛋白(CDHE)ELISA试剂盒 ELISA Kit for Cadherin, Epithelial (CDHE) 检测范围:14.326-1000pg/ml
xyA032Ga 鸡酸性成纤维细胞生长因子(FGF1)ELISA试剂盒 ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1) 检测范围:14.326-1000pg/ml
xyA009Ga 鸡血管生成素2(ANGPT2)ELISA试剂盒 ELISA Kit for Angiopoietin 2 (ANGPT2) 检测范围:14.326-1000pg/ml
xyA034Ga 鸡成纤维细胞生长因子4(FGF4)ELISA试剂盒 ELISA Kit for Fibroblast Growth Factor 4 (FGF4) 检测范围:14.326-1000pg/ml
xyA036Ga 鸡成纤维细胞生长因子9(FGF9)ELISA试剂盒 ELISA Kit for Fibroblast Growth Factor 9 (FGF9) 检测范围:14.326-1000pg/ml
xyA605Ga 鸡脂联素(ADP)ELISA试剂盒 ELISA Kit for Adiponectin (ADP) 检测范围:14.326-1000pg/ml
xyA543Ga 鸡免疫球蛋白M(IgM)ELISA试剂盒 ELISA Kit for Immunoglobulin M (IgM) 检测范围:14.326-1000pg/ml
xyA380Ga 鸡血管活性肠肽(VIP)ELISA试剂盒 ELISA Kit for Vasoactive Intestinal Peptide (VIP) 检测范围:14.326-1000pg/ml
xyB827Ga 鸡轴突生长诱向因子1(Ntn1)ELISA试剂盒 ELISA Kit for Netrin 1 (Ntn1) 检测范围:14.326-1000pg/ml
xyA991Ga 鸡饥饿素(GHRL)ELISA试剂盒 ELISA Kit for Ghrelin (GHRL) 检测范围:14.326-1000pg/ml
xyA005Ga 鸡血管紧张素Ⅱ(ANGⅡ)ELISA试剂盒 ELISA Kit for Angiotensin II (AngII) 检测范围:14.326-1000pg/ml
xyA861Ga 鸡补体成分3(C3)ELISA试剂盒 ELISA Kit for Complement Component 3 (C3) 检测范围:14.326-1000pg/ml
xyB480Ga 鸡甘露糖结合蛋白(MBL)ELISA试剂盒 ELISA Kit for Mannose Binding Lectin (MBL) 检测范围:14.326-1000pg/ml
xyA641Ga 鸡分泌型免疫球蛋白A(sIgA)ELISA试剂盒 ELISA Kit for Secretory Immunoglobulin A (sIgA) 检测范围:14.326-1000pg/ml
xyA873Ga 鸡热休克蛋白70(HSP70)ELISA试剂盒 ELISA Kit for Heat Shock Protein 70 (HSP70) 检测范围:14.326-1000pg/ml
xyA053Ga 鸡胰岛素样生长因子结合蛋白2(IGFBP2)ELISA试剂盒 ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2) 检测范围:14.326-1000pg/ml
xyA471Ga 鸡骨钙素(OC)ELISA试剂盒 ELISA Kit for Osteocalcin (OC) 检测范围:14.326-1000pg/ml
xyA073Ga 鸡白介素2(IL2)ELISA试剂盒 ELISA Kit for Interleukin 2 (IL2) 检测范围:14.326-1000pg/ml
xyA077Ga 鸡白介素4(IL4)ELISA试剂盒 ELISA Kit for Interleukin 4 (IL4) 检测范围:14.326-1000pg/ml
下一篇:人胰岛素样生长因子-2检测试剂盒