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简要描述:人胰岛素样生长因子结合蛋白1检测试剂盒适用于体外定量检测人血清、血浆或其它相关生物液体中天然和重组胰岛素样生长因子结合蛋白1的浓度。本试剂盒仅供体外研究使用、不用于临床诊断!
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所属分类:Human/人检测试剂盒
更新时间:2015-11-05
人胰岛素样生长因子结合蛋白1检测试剂盒在ELISA检测系统的关键要素中,包被原的性质很重要,蛋白浓度,是否降解,这关系到你做出的抗体可不可以被其识别,所以保留抗原很重要,ELISA试剂盒我做重组蛋白时,一定要在冰浴下缓慢融化就是这个道理。封锁就是让大量不相关的蛋白质充填这些旷地空闲,从而排斥ELISA后的步骤中干扰物质的再吸附。但有时因为试验的需要,包被原的特殊性也可能采用中性的缓冲溶液来包。
【中文名称】人胰岛素样生长因子结合蛋白1检测试剂盒【常用名称】人胰岛素样生长因子结合蛋白1ELISA试剂盒【规格】96T/48T【品牌】TSZ【价格】电议【保存】2-8℃,避光保存【说明书】随货发送或直接销售人员索取【有效期】六个月【检测目的】用于测定血清,血浆及相关液体等样本,例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本
注:本产品仅供科研使用,不得用于临床诊断
人胰岛素样生长因子结合蛋白1检测试剂盒实验内容与方法
1.在微量反应板每孔加入待检标本50μl,设阳性、阴性对照各2孔,每孔加入阳性(或阴性)对照各1滴,并设空白对照1孔。
2.每孔加入酶结合物1滴(空白对照除外),充分混匀,封板,置37℃孵育30分钟。
3.弃去孔内液体,洗涤液注满各孔,静置5秒,甩干,重复5次后拍干。
4.每孔加显色剂A液、B液各1滴,充分混匀,封板,置37℃孵育15分钟。
5.每孔加终止液1滴,充分混匀。
6.用酶标仪读数,取波长450nm,先用空白孔校零,然后读取各孔OD值。
人胰岛素样生长因子结合蛋白1检测试剂盒相关现货试剂盒如下:xyA104Ga 鸡胆囊收缩素A受体(CCKAR)ELISA试剂盒 ELISA Kit for Cholecystokinin A Receptor (CCKAR) 检测范围:14.326-1000pg/ml
xyA109Ga 鸡肌肌酸激酶(CKM)ELISA试剂盒 ELISA Kit for Creatine Kinase, Muscle (CKM) 检测范围:14.326-1000pg/ml
xyA052Ga 鸡胰岛素样生长因子结合蛋白1(IGFBP1)ELISA试剂盒 ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1) 检测范围:14.326-1000pg/ml
xyA215Ga 鸡Ⅰ型胶原α2(COL1α2)ELISA试剂盒 ELISA Kit for Collagen Type I Alpha 2 (COL1a2) 检测范围:14.326-1000pg/ml
xyA822Ga 鸡60kD热休克蛋白(HSPD1)ELISA试剂盒 ELISA Kit for Heat Shock 60kD Protein 1, Chaperonin (HSPD1) 检测范围:14.326-1000pg/ml
xyA871Ga 鸡热休克蛋白β2(HSPβ2)ELISA试剂盒 ELISA Kit for Heat Shock Protein Beta 2 (HSPb2) 检测范围:14.326-1000pg/ml
xyA782Ga 鸡碳酸酐酶Ⅱ(CA2)ELISA试剂盒 ELISA Kit for Carbonic Anhydrase II (CA2) 检测范围:14.326-1000pg/ml
xyA855Ga 鸡核因子κB受体激活因子配基(RANκL)ELISA试剂盒 ELISA Kit for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) 检测范围:14.326-1000pg/ml
xyA062Ga 鸡白介素16(IL16)ELISA试剂盒 ELISA Kit for Interleukin 16 (IL16) 检测范围:14.326-1000pg/ml
xyB266Ga 鸡胰高血糖素(GC)ELISA试剂盒 ELISA Kit for Glucagon (GC) 检测范围:14.326-1000pg/ml
xyB193Ga 鸡*(LZM)ELISA试剂盒 ELISA Kit for Lysozyme (LZM) 检测范围:14.326-1000pg/ml
xyA084Ga 鸡瘦素(LEP)ELISA试剂盒 ELISA Kit for Leptin (LEP) 检测范围:14.326-1000pg/ml
xyA058Ga 鸡白介素12B(IL12B)ELISA试剂盒 ELISA Kit for Interleukin 12B (IL12B) 检测范围:14.326-1000pg/ml
xyA080Ga 鸡白介素8(IL8)ELISA试剂盒 ELISA Kit for Interleukin 8 (IL8) 检测范围:14.326-1000pg/ml
xyB653Ga 鸡肌肉生长抑制素(MSTN)ELISA试剂盒 ELISA Kit for Myostatin (MSTN) 检测范围:14.326-1000pg/ml
xyB119Ga 鸡金属硫蛋白1(MT1)ELISA试剂盒 ELISA Kit for Metallothionein 1 (MT1) 检测范围:14.326-1000pg/ml
xyA056Ga 鸡白介素10(IL10)ELISA试剂盒 ELISA Kit for Interleukin 10 (IL10) 检测范围:14.326-1000pg/ml
xyA105Ga 鸡神经生长因子(NGF)ELISA试剂盒 ELISA Kit for Nerve Growth Factor (NGF) 检测范围:14.326-1000pg/ml
xyA106Ga 鸡神经营养因子3(NT3)ELISA试剂盒 ELISA Kit for Neurotrophin 3 (NT3) 检测范围:14.326-1000pg/ml
xyA002Ga 鸡白细胞激活黏附因子(ALCAM)ELISA试剂盒 ELISA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM) 检测范围:14.326-1000pg/ml
xyA112Ga 鸡β细胞素(βTC)ELISA试剂盒 ELISA Kit for Betacellulin (bTC) 检测范围:14.326-1000pg/ml
xyC011Ga 鸡补体因子B(CFB)ELISA试剂盒 ELISA Kit for Complement Factor B (CFB) 检测范围:14.326-1000pg/ml
xyA118Ga 鸡CD30配体(CD30L)ELISA试剂盒 ELISA Kit for Cluster Of Differentiation 30 Ligand (CD30L) 检测范围:14.326-1000pg/ml
xyA119Ga 鸡CD40配体(CD40L)ELISA试剂盒 ELISA Kit for Cluster Of Differentiation 40 Ligand (CD40L) 检测范围:14.326-1000pg/ml
xyA120Ga 鸡干细胞因子(SCF)ELISA试剂盒 ELISA Kit for Stem Cell Factor (SCF) 检测范围:14.326-1000pg/ml
xyA006Ga 鸡双调蛋白(AREG)ELISA试剂盒 ELISA Kit for Amphiregulin (AREG) 检测范围:14.326-1000pg/ml
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