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简要描述:人胰岛素样生长因子结合蛋白-3检测试剂盒适用于体外定量检测人血清、血浆或其它相关生物液体中天然和重组胰岛素样生长因子结合蛋白-3的浓度。本试剂盒仅供体外研究使用、不用于临床诊断!
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所属分类:Human/人检测试剂盒
更新时间:2015-11-05
人胰岛素样生长因子结合蛋白-3检测试剂盒在ELISA检测系统的关键要素中,包被原的性质很重要,蛋白浓度,是否降解,这关系到你做出的抗体可不可以被其识别,所以保留抗原很重要,ELISA试剂盒我做重组蛋白时,一定要在冰浴下缓慢融化就是这个道理。封锁就是让大量不相关的蛋白质充填这些旷地空闲,从而排斥ELISA后的步骤中干扰物质的再吸附。但有时因为试验的需要,包被原的特殊性也可能采用中性的缓冲溶液来包。
【中文名称】人胰岛素样生长因子结合蛋白-3检测试剂盒【常用名称】人胰岛素样生长因子结合蛋白-3ELISA试剂盒【规格】96T/48T【品牌】TSZ【价格】电议【保存】2-8℃,避光保存【说明书】随货发送或直接销售人员索取【有效期】六个月【检测目的】用于测定血清,血浆及相关液体等样本,例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本
注:本产品仅供科研使用,不得用于临床诊断
人胰岛素样生长因子结合蛋白-3检测试剂盒实验内容与方法
1.在微量反应板每孔加入待检标本50μl,设阳性、阴性对照各2孔,每孔加入阳性(或阴性)对照各1滴,并设空白对照1孔。
2.每孔加入酶结合物1滴(空白对照除外),充分混匀,封板,置37℃孵育30分钟。
3.弃去孔内液体,洗涤液注满各孔,静置5秒,甩干,重复5次后拍干。
4.每孔加显色剂A液、B液各1滴,充分混匀,封板,置37℃孵育15分钟。
5.每孔加终止液1滴,充分混匀。
6.用酶标仪读数,取波长450nm,先用空白孔校零,然后读取各孔OD值。
人胰岛素样生长因子结合蛋白-3检测试剂盒相关现货试剂盒如下:xyA471Rb 兔骨钙素(OC)ELISA试剂盒 ELISA Kit for Osteocalcin (OC) 检测范围:12.198-1000pg/ml
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xyA002Rb 兔白细胞激活黏附因子(ALCAM)ELISA试剂盒 ELISA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM) 检测范围:12.198-1000pg/ml
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xyC011Rb 兔补体因子B(CFB)ELISA试剂盒 ELISA Kit for Complement Factor B (CFB) 检测范围:12.198-1000pg/ml
xyA117Rb 兔胞外超氧化物歧化酶(SOD3)ELISA试剂盒 ELISA Kit for Superoxide Dismutase 3, Extracellular (SOD3) 检测范围:12.198-1000pg/ml
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xyA128Rb 兔组织金属蛋白酶抑制因子2(TIMP2)ELISA试剂盒 ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) 检测范围:12.198-1000pg/ml
xyA006Rb 兔双调蛋白(AREG)ELISA试剂盒 ELISA Kit for Amphiregulin (AREG) 检测范围:12.198-1000pg/ml
xyA214Rb 兔窖蛋白(CAV1)ELISA试剂盒 ELISA Kit for Caveolin 1 (CAV1) 检测范围:12.198-1000pg/ml
xyA131Rb 兔转化生长因子β诱导蛋白(TGFβI)ELISA试剂盒 ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI) 检测范围:12.198-1000pg/ml
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xyA134Rb 兔肿瘤坏死因子β(TNFβ)ELISA试剂盒 ELISA Kit for Tumor Necrosis Factor Beta (TNFb) 检测范围:12.198-1000pg/ml
xyA061Rb 兔白介素15(IL15)ELISA试剂盒 ELISA Kit for Interleukin 15 (IL15) 检测范围:12.198-1000pg/ml
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xyA178Rb 兔α2-HS糖蛋白(αHSG)ELISA试剂盒 ELISA Kit for Alpha-2-Heremans Schmid Glycoprotein (aHSG) 检测范围:12.198-1000pg/ml
xyA164Rb 兔泛素(Ub)ELISA试剂盒 ELISA Kit for Ubiquitin (Ub) 检测范围:12.198-1000pg/ml
xyA227Rb 兔亲环素B(CYPB)ELISA试剂盒 ELISA Kit for Cyclophilin B (CYPB) 检测范围:12.198-1000pg/ml
xyA874Rb 兔缓激肽(BK)ELISA试剂盒 ELISA Kit for Bradykinin (BK) 检测范围:12.198-1000pg/ml
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xyA267Rb 兔组织蛋白酶K(CTSK)ELISA试剂盒 ELISA Kit for Cathepsin K (CTSK) 检测范围:12.198-1000pg/ml
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