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简要描述:人胰岛素样生长因子结合蛋白4检测试剂盒适用于体外定量检测人血清、血浆或其它相关生物液体中天然和重组胰岛素样生长因子结合蛋白4的浓度。本试剂盒仅供体外研究使用、不用于临床诊断!
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所属分类:Human/人检测试剂盒
更新时间:2015-11-05
人胰岛素样生长因子结合蛋白4检测试剂盒在ELISA检测系统的关键要素中,包被原的性质很重要,蛋白浓度,是否降解,这关系到你做出的抗体可不可以被其识别,所以保留抗原很重要,ELISA试剂盒我做重组蛋白时,一定要在冰浴下缓慢融化就是这个道理。封锁就是让大量不相关的蛋白质充填这些旷地空闲,从而排斥ELISA后的步骤中干扰物质的再吸附。但有时因为试验的需要,包被原的特殊性也可能采用中性的缓冲溶液来包。
【中文名称】人胰岛素样生长因子结合蛋白4检测试剂盒【常用名称】人胰岛素样生长因子结合蛋白4ELISA试剂盒【规格】96T/48T【品牌】TSZ【价格】电议【保存】2-8℃,避光保存【说明书】随货发送或直接销售人员索取【有效期】六个月【检测目的】用于测定血清,血浆及相关液体等样本,例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本
注:本产品仅供科研使用,不得用于临床诊断
人胰岛素样生长因子结合蛋白4检测试剂盒实验内容与方法
1.在微量反应板每孔加入待检标本50μl,设阳性、阴性对照各2孔,每孔加入阳性(或阴性)对照各1滴,并设空白对照1孔。
2.每孔加入酶结合物1滴(空白对照除外),充分混匀,封板,置37℃孵育30分钟。
3.弃去孔内液体,洗涤液注满各孔,静置5秒,甩干,重复5次后拍干。
4.每孔加显色剂A液、B液各1滴,充分混匀,封板,置37℃孵育15分钟。
5.每孔加终止液1滴,充分混匀。
6.用酶标仪读数,取波长450nm,先用空白孔校零,然后读取各孔OD值。
人胰岛素样生长因子结合蛋白4检测试剂盒相关现货试剂盒如下:xyA080Rb 兔白介素8(IL8)ELISA试剂盒 ELISA Kit for Interleukin 8 (IL8) 检测范围:12.198-1000pg/ml
xyA087Rb 兔单核细胞趋化蛋白1(MCP1)ELISA试剂盒 ELISA Kit for Monocyte Chemotactic Protein 1 (MCP1) 检测范围:12.198-1000pg/ml
xyA049Rb 兔干扰素γ(IFNγ)ELISA试剂盒 ELISA Kit for Interferon Gamma (IFNg) 检测范围:12.198-1000pg/ml
xyA047Rb 兔肝细胞生长因子(HGF)ELISA试剂盒 ELISA Kit for Hepatocyte Growth Factor (HGF) 检测范围:12.198-1000pg/ml
xyA553Rb 兔基质金属蛋白酶9(MMP9)ELISA试剂盒 ELISA Kit for Matrix Metalloproteinase 9 (MMP9) 检测范围:12.198-1000pg/ml
xyA552Rb 兔组织金属蛋白酶抑制因子1(TIMP1)ELISA试剂盒 ELISA Kit for Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) 检测范围:12.198-1000pg/ml
xyA551Rb 兔碱性成纤维细胞生长因子(FGF2)ELISA试剂盒 ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2) 检测范围:12.198-1000pg/ml
xyA547Rb 兔血管细胞粘附分子1(VCAM1)ELISA试剂盒 ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1) 检测范围:12.198-1000pg/ml
xyA544Rb 兔免疫球蛋白G(IgG)ELISA试剂盒 ELISA Kit for Immunoglobulin G (IgG) 检测范围:12.198-1000pg/ml
xyA543Rb 兔免疫球蛋白M(IgM)ELISA试剂盒 ELISA Kit for Immunoglobulin M (IgM) 检测范围:12.198-1000pg/ml
xyA482Rb 兔内皮素1(EDN1)ELISA试剂盒 ELISA Kit for Endothelin 1 (EDN1) 检测范围:12.198-1000pg/ml
xyA084Rb 兔瘦素(LEP)ELISA试剂盒 ELISA Kit for Leptin (LEP) 检测范围:12.198-1000pg/ml
xyA548Rb 兔细胞间粘附分子1(ICAM1)ELISA试剂盒 ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1) 检测范围:12.198-1000pg/ml
xyA534Rb 兔细胞间粘附分子2(ICAM2)ELISA试剂盒 ELISA Kit for Intercellular Adhesion Molecule 2 (ICAM2) 检测范围:12.198-1000pg/ml
xyA532Rb 兔纤溶酶原激活物抑制因子1(PAI1)ELISA试剂盒 ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1) 检测范围:12.198-1000pg/ml
xyA531Rb 兔纤溶酶原激活物抑制因子2(PAI2)ELISA试剂盒 ELISA Kit for Plasminogen Activator Inhibitor 2 (PAI2) 检测范围:12.198-1000pg/ml
xyA529Rb 兔血栓调节蛋白(TM)ELISA试剂盒 ELISA Kit for Thrombomodulin (TM) 检测范围:12.198-1000pg/ml
xyA528Rb 兔血小板衍生生长因子A(PDGFA)ELISA试剂盒 ELISA Kit for Plaet Derived Growth Factor Subunit A (PDGFA) 检测范围:12.198-1000pg/ml
xyA448Rb 兔胰岛素(INS)ELISA试剂盒 ELISA Kit for Insulin (INS) 检测范围:12.198-1000pg/ml
xyA050Rb 兔胰岛素样生长因子1(IGF1)ELISA试剂盒 ELISA Kit for Insulin Like Growth Factor 1 (IGF1) 检测范围:12.198-1000pg/ml
xyA051Rb 兔胰岛素样生长因子2(IGF2)ELISA试剂盒 ELISA Kit for Insulin Like Growth Factor 2 (IGF2) 检测范围:12.198-1000pg/ml
xyA889Rb 兔肾素(REN)ELISA试剂盒 ELISA Kit for Renin (REN) 检测范围:12.198-1000pg/ml
xyA524Rb 兔组织因子(TF)ELISA试剂盒 ELISA Kit for Tissue Factor (TF) 检测范围:12.198-1000pg/ml
xyA029Rb 兔E选择素(SELE)ELISA试剂盒 ELISA Kit for Selectin, Endothelium (SELE) 检测范围:12.198-1000pg/ml
xyA033Rb 兔干扰素α(IFNα)ELISA试剂盒 ELISA Kit for Interferon Alpha (IFNa) 检测范围:12.198-1000pg/ml
xyA225Rb 兔心钠肽(ANP)ELISA试剂盒 ELISA Kit for Atrial Natriuretic Peptide (ANP) 检测范围:12.198-1000pg/ml
xyA530Rb 兔纤维蛋白原降解产物(FDP)ELISA试剂盒 ELISA Kit for Fibrinogen Degradation Product (FDP) 检测范围:12.198-1000pg/ml
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