全国统一服务热线:15221858802
简要描述:人*检测试剂盒适用于体外定量检测人血清、血浆或其它相关生物液体中天然和重组*的浓度。本试剂盒仅供体外研究使用、不用于临床诊断!
产品型号:
所属分类:Human/人检测试剂盒
更新时间:2015-11-05
人*检测试剂盒在ELISA检测系统的关键要素中,包被原的性质很重要,蛋白浓度,是否降解,这关系到你做出的抗体可不可以被其识别,所以保留抗原很重要,ELISA试剂盒我做重组蛋白时,一定要在冰浴下缓慢融化就是这个道理。封锁就是让大量不相关的蛋白质充填这些旷地空闲,从而排斥ELISA后的步骤中干扰物质的再吸附。但有时因为试验的需要,包被原的特殊性也可能采用中性的缓冲溶液来包。
【中文名称】人*检测试剂盒【常用名称】人*ELISA试剂盒【规格】96T/48T【品牌】TSZ【价格】电议【保存】2-8℃,避光保存【说明书】随货发送或直接销售人员索取【有效期】六个月【检测目的】用于测定血清,血浆及相关液体等样本,例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、脑脊液、细胞培养上清、组织匀浆等标本
注:本产品仅供科研使用,不得用于临床诊断
实验内容与方法
1.在微量反应板每孔加入待检标本50μl,设阳性、阴性对照各2孔,每孔加入阳性(或阴性)对照各1滴,并设空白对照1孔。
2.每孔加入酶结合物1滴(空白对照除外),充分混匀,封板,置37℃孵育30分钟。
3.弃去孔内液体,洗涤液注满各孔,静置5秒,甩干,重复5次后拍干。
4.每孔加显色剂A液、B液各1滴,充分混匀,封板,置37℃孵育15分钟。
5.每孔加终止液1滴,充分混匀。
6.用酶标仪读数,取波长450nm,先用空白孔校零,然后读取各孔OD值。
相关现货试剂盒如下:xyB036Mu 小鼠生长激素受体(GHR)检测试剂盒 ELISA Kit for Growth Hormone Receptor (GHR) 检测范围: 11.254-1000pg/ml
xyB081Mu 小鼠70kDa热休克蛋白1A(HSPA1A)检测试剂盒 ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A) 检测范围: 11.254-1000pg/ml
xyC559Mu 小鼠吻素1(KISS1)检测试剂盒 ELISA Kit for Kisspeptin 1 (KISS1) 检测范围: 11.254-1000pg/ml
xyC628Mu 小鼠微精蛋白β(MSMβ)检测试剂盒 ELISA Kit for Microseminoprotein Beta (MSMb) 检测范围: 11.254-1000pg/ml
xyC795Mu 小鼠跨膜*蛋白酶2(TMPRSS2)检测试剂盒 ELISA Kit for Transmembrane Protease, Serine 2 (TMPRSS2) 检测范围: 11.254-1000pg/ml
xyC805Mu 小鼠肿廇蛋白P63(TP63)检测试剂盒 ELISA Kit for Tumor Protein P63 (TP63) 检测范围: 11.254-1000pg/ml
xyD050Mu 小鼠γ-内啡肽(γEP)检测试剂盒 ELISA Kit for Gamma-Endorphin (gEP) 检测范围: 11.254-1000pg/ml
xyL824Mu 小鼠肾脏脑蛋白(KIBRA)检测试剂盒 ELISA Kit for Kidney And Brain Protein (KIBRA) 检测范围: 11.254-1000pg/ml
xyD318Mu 小鼠左右决定因子2(LEFTY2)检测试剂盒 ELISA Kit for Left/Right Determination Factor 2 (LEFTY2) 检测范围: 11.254-1000pg/ml
xyD774Mu 小鼠蛋白二硫化物异构酶A4(PDIA4)检测试剂盒 ELISA Kit for Protein Disulfide Isomerase A4 (PDIA4) 检测范围: 11.254-1000pg/ml
xyD827Mu 小鼠ⅡA组磷脂酶A2(PLA2G2A)检测试剂盒 ELISA Kit for Phospholipase A2, Group IIA (PLA2G2A) 检测范围: 11.254-1000pg/ml
xyE658Mu 小鼠细胞分裂周期因子25(CDC25)检测试剂盒 ELISA Kit for Cell Division Cycle Protein 25 (CDC25) 检测范围: 11.254-1000pg/ml
xyE771Mu 小鼠白介素11受体α(IL11Rα)检测试剂盒 ELISA Kit for Interleukin 11 Receptor Alpha (IL11Ra) 检测范围: 11.254-1000pg/ml
xyH375Mu 小鼠PHD指蛋白8(PHF8)检测试剂盒 ELISA Kit for PHD Finger Protein 8 (PHF8) 检测范围: 11.254-1000pg/ml
xyH428Mu 小鼠异戊烯半*氧化酶1(PCYOX1)检测试剂盒 ELISA Kit for Prenylcysteine Oxidase 1 (PCYOX1) 检测范围: 11.254-1000pg/ml
xyH665Mu 小鼠母系胚胎*拉链蛋白激酶(MELK)检测试剂盒 ELISA Kit for Maternal Embryonic Leucine Zipper Kinase (MELK) 检测范围: 11.254-1000pg/ml
xyG342Mu 小鼠色胺酸羟化酶1(TPH1)检测试剂盒 ELISA Kit for Tryptophan Hydroxylase 1 (TPH1) 检测范围: 11.254-1000pg/ml
xyL292Mu 小鼠含TCP1伴侣蛋白亚基2(CCT2)检测试剂盒 ELISA Kit for Chaperonin Containing TCP1, Subunit 2 (CCT2) 检测范围: 11.254-1000pg/ml
xyG121Mu 小鼠淀粉样蛋白β前体样蛋白1(APLP1)检测试剂盒 ELISA Kit for Amyloid Beta Precursor Like Protein 1 (APLP1) 检测范围: 11.254-1000pg/ml
xyS094Mu 小鼠免疫球蛋白G2α(IgG2α)检测试剂盒 ELISA Kit for Immunoglobulin G2a (IgG2a) 检测范围: 11.254-1000pg/ml
xyE916Mu 小鼠沉默调节蛋白6(SIRT6)检测试剂盒 ELISA Kit for Sirtuin 6 (SIRT6) 检测范围: 11.254-1000pg/ml
xyF419Mu 小鼠硬脂酰*去饱和酶(SCD)检测试剂盒 ELISA Kit for Stearoyl Coenzyme A Desaturase (SCD) 检测范围: 11.254-1000pg/ml
xyF180Mu 小鼠17-β-羟基类固醇脱氢酶10(HSD17β10)检测试剂盒 ELISA Kit for 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) 检测范围: 11.254-1000pg/ml
xyA747Mu 小鼠补体1q(C1q)检测试剂盒 ELISA Kit for Complement 1q (C1q) 检测范围: 11.254-1000pg/ml
xyB092Mu 小鼠骨涎蛋白(BSP)检测试剂盒 ELISA Kit for Bone Sialoprotein (BSP) 检测范围: 11.254-1000pg/ml
xyB331Mu 小鼠套膜蛋白(iNV)检测试剂盒 ELISA Kit for Involucrin (iNV) 检测范围: 11.254-1000pg/ml
xyC020Mu 小鼠S100钙结合蛋白A4(S100A4)检测试剂盒 ELISA Kit for S100 Calcium Binding Protein A4 (S100A4) 检测范围: 11.254-1000pg/ml
下一篇:人胰高血糖素样肽检测试剂盒